General Stability | Organism |
---|---|
bovine serum albumin stabilizes dilute enzyme solutions | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at 0.1 mM, slight | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.1, 30°C, addition of Ca2+/calmodulin increases the Km 2fold | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at physiological concentrations | Rattus norvegicus | |
Ca2+ | activation by Ca2+/calmodulin | Rattus norvegicus | |
Ca2+ | not activated by free Ca2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
1 * 53000, SDS-PAGE | Rattus norvegicus |
53000 | - |
2 * 53000, native enzyme consists of a dimer of 2 catalytic subunits with 1 calmodulin molecule associated with each catalytic subunit, SDS-PAGE | Rattus norvegicus |
70000 | - |
gel filtration | Rattus norvegicus |
150000 | 160000 | in the presence of Ca2+/calmodulin, gel filtration, the interaction of calmodulin with the monomeric catalytic subunit promotes dimerization of the enzyme | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | important regulatory role in inositol phosphate signaling by promoting the formation of additional inositol polyphosphate isomers | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
adult | - |
Rattus norvegicus | - |
Sprague-Dawley or Lewis rats | - |
Purification (Comment) | Organism |
---|---|
2700fold | Rattus norvegicus |
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | - |
30°C | Rattus norvegicus |
3.5 | - |
30°C, fully activated enzyme in presence of Ca2+/calmodulin | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, 20 mM Tris-HCl buffer, pH 7.2, 1 mM Mg(CH3COO)2, 1 mM DTT, 0.1 mM EGTA, 0.5 mg/ml bovine serum albumin, 3 months, less than 20% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | important regulatory role in inositol phosphate signaling by promoting the formation of additional inositol polyphosphate isomers | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, native enzyme consists of a dimer of 2 catalytic subunits with 1 calmodulin molecule associated with each catalytic subunit, SDS-PAGE | Rattus norvegicus |
monomer | 1 * 53000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about half-maximal activity at pH 7 and about 60% of maximal activity at pH 9 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP-dependent | Rattus norvegicus | |
Calmodulin | activation by Ca2+/calmodulin | Rattus norvegicus |